4-Dec-2025
New ‘PLAMseq’ method revolutionizes the study of genome proteins
University of SevillePeer-Reviewed Publication
A team at CABIMER has developed PLAMseq, a technique that for the first time enables the simultaneous identification of chromatin-associated proteins and their precise genomic locations within a single experiment, overcoming limitations of traditional methods like ChIP-seq and mass spectrometry that require complex workflows and specific antibodies. By leveraging rapid TurboID-mediated biotinylation, PLAMseq uncovers previously unknown molecular interactions—such as SETDB1 binding to SUMO-modified histones—and demonstrates strong performance on key genome regulators including CTCF, RNA polymerase II, and modified H1 histones. This integrated proteogenomic approach opens new avenues for investigating epigenetic mechanisms underlying cancer and neurological or developmental disorders, potentially accelerating the discovery of therapeutic targets and biomarkers while positioning Andalusian research groups at the forefront of chromatin biology innovation.
- Journal
- Science Advances