F. nucleatum promotes triple-negative breast cancer invasion and metastasis in vivo and in vitro (IMAGE)
Caption
(A) Schematic diagram of animal studies. BALB/c mice were implanted with 4T1 mouse breast cancer cells on day 0 and intratumorally injected with F. nucleatum or PBS from day 7 to the end of the experiment. (B) Representative images of tumors under different treatments (n = 5). (C) Tumor weight of mice in each group (n = 5). (D) Number of metastatic nodules in the lungs in each group (n = 5). (E) Hematoxylin–eosin staining of the lung metastatic area. (F) Representative images of immunohistochemistry-stained E-Cadherin and N-Cadherin in paraffin-embedded tumor tissues. Cell nuclei were stained with hematoxylin. Scale bar, 40 μm. (G) Representative images of Transwell assays. Scale bar, 50 μm. (H) Representative flow cytometry data of CD8+ T cells in tumor tissues. (I) Quantification of CD8+ T cells in tumor tissues. (J) Representative flow cytometry data of Ki67+CD8+ T cells in tumor tissues. (K) Quantification of Ki67+CD8+ T cells in tumor tissues. (L) Representative flow cytometry data of IFN-γ+CD8+ T cells in tumor tissues. (M) Quantification of IFN-γ+CD8+ T cells in tumor tissues. All data are presented as the mean ± standard deviation of triplicate experiments. P-values were analyzed by a two-tailed unpaired Student's t-test. P-values less than 0.05 were considered statistically significant. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001.
Credit
Qian Zeng, Yan Ye, Kejia Wu, Kexin Wang, Xing Zhou, Wenjing Zhou, Shiqiao Zhao, Tao Zhang, Tingmei Chen
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