F. nucleatum activates tryptophan metabolism in triple-negative breast cancer (TNBC) cells and promotes cell invasion (IMAGE)
Caption
(A) Schematic diagram of tryptophan metabolism. (B) The expression of IL4I1 was measured by Western blotting in TNBC cells treated with F. nucleatum or F. nucleatum plus ruxolitinib. (C) I3P in supernatants of MDA-MB-231 cells. (D) IAA in supernatants and intracellular of MDA-MB-231 cells. (E) I3A in supernatants of MDA-MB-231 cells. (F) ILA in supernatants and intracellular of MDA-MB-231 cells. (G) The mRNA levels of AHR downstream genes were measured by quantitative PCR analysis in MDA-MB-231 cells and BT549 cells treated with control and I3P. (H) Representative images of Transwell assays. Scale bar, 50 μm. (I) The expression of PD-L1 was measured by Western blotting in TNBC cells treated with control or 25 μM I3P for 4 h. All data are presented as the mean ± standard deviation of triplicate experiments. P-values were analyzed by one-way ANOVA (C–F) or two-tailed unpaired Student's t-test (G, H). P-values less than 0.05 were considered statistically significant. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001.
Credit
Qian Zeng, Yan Ye, Kejia Wu, Kexin Wang, Xing Zhou, Wenjing Zhou, Shiqiao Zhao, Tao Zhang, Tingmei Chen
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