IL-1β promotes KRT7 expression through p65. (A) The intersection between differentially expressed mRNA and protein. (IMAGE)
Caption
(B, C) The expression of KRT7, CPA4, SLC2A1, and TNFSF6B mRNA was detected by quantitative real-time PCR after using IL1β (3 ng/mL) or Anakinra (50 nM). (D) The protein level of KRT7 was measured by Western blotting after using IL1β (3 ng/mL) or Anakinra (50 nM). (F–I) After using IL1β (3 ng/mL) for colorectal cancer (CRC) cells for 48 h, HCT8 and HCT116 cells were treated with cycloheximide (CHX 20 nM). Total protein was harvested at different time points, and KRT7 protein expression was measured by Western blotting and quantified by densitometry. (J–L) The CUT-Tag assay indicated that p65 bound to the TBS1 regions in the KRT7 promoter in CRC cells. (M, N) Luciferase reporter assay was used to detect luciferase activity of CRC cells after co-transfecting KRT7 plasmids, Renilla plasmids, and p65 plasmids.
Credit
Zhenzhou Chen, Kuan He, Dong Peng, Donghui Gao, Hongyu Zhang, Xiaolong Liang, Xiang Xu, Dongli Du, Luyue Wang, Li Zeng, Zongyue Zeng, Xingye Wu
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