Maternal infections significantly affect cardiac metabolism in offspring mice. (IMAGE)
Caption
Pregnant mice were intraperitoneally injected with LPS (300 μg/kg) or Poly(I:C) (20 mg/kg) at E16.5. The hearts of the newborn offspring mice were extracted within 12 h of birth [postnatal day (P)0] for mRNA sequencing and bioinformatic analysis. (A, B) KEGG pathway classification histograms of differentially expressed genes in LPS versus control and Poly(I:C) versus control. The horizontal axis represents functional categories, while the vertical axis shows the number of genes within each category, with light colors representing differential genes and dark colors representing all genes. The left vertical axis shows the proportion of genes annotated to that function (differential genes/all genes). (C) Heatmap of metabolism-related genes among control, LPS, and Poly(I:C). (D) Violin plots of expression of metabolism-related genes among control, LPS, and Poly(I:C). (E) Volcano plot of differentially expressed genes in LPS versus control. (F) Volcano plot of differentially expressed genes in Poly(I:C) versus control. (G) Violin plots of expression of mitochondrial-related genes among control, LPS, and Poly(I:C). One-way analysis of variance (ANOVA) followed by Holm-Sidak post-test for multiple comparisons was used for comparing more than two groups. The data were presented as mean ± standard error.
Credit
Yingrui Li, Willfredius Mugishagwe Rutahoile, Binquan Xiong, Jianlin Du, Songbai Deng, Bin Liu, Xiaodong Jing, Huiping Yang, Yue Han, Qiang She
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