Poly(I:C) challenge, but not LPS, impairs mitochondrial function in the cardiac differentiation process by increasing lipid peroxidation levels. (IMAGE)
Caption
From day 16 to day 20 of hiPSC differentiation into cardiomyocytes, LPS (5 μg/mL), Poly(I:C) (10 μg/mL), and Fer-1 (2 μM) treatments were applied. On day 20 of differentiation, cells were used for the experiments. (A) Representative images of hiPSC-B1-CM and hiPSC-F1-CM cell lines stained with 4-HNE/TNNT2/DAPI (bar = 20 μm). (B) Malonaldehyde (MDA) content in hiPSC-B1-CM and hiPSC-F1-CM cell lines with or without LPS or Poly(I:C) challenge. (C) Glutathione disulfide (GSSG) in hiPSC-B1-CM and hiPSC-F1-CM cell lines with or without LPS or Poly(I:C) challenge. (D) Total glutathione (T-GSH)/GSSG in hiPSC-B1-CM and hiPSC-F1-CM cell lines with or without LPS or Poly(I:C) challenge. (E) ATP content in hiPSC-B1-CM and hiPSC-F1-CM cell lines in each group. (F) Ts2 to nuclear B2M DNA ratio in hiPSC-B1-CM and hiPSC-F1-CM cell lines in each group. One-way analysis of variance (ANOVA) followed by Holm-Sidak post-test for multiple comparisons was used for comparing more than two groups. The data were presented as mean ± standard error.
Credit
Yingrui Li, Willfredius Mugishagwe Rutahoile, Binquan Xiong, Jianlin Du, Songbai Deng, Bin Liu, Xiaodong Jing, Huiping Yang, Yue Han, Qiang She
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