NAC counteracts the effects of LPS and Poly(I:C) on the hearts of offspring mice and hiPSC-CM differentiation. (IMAGE)
Caption
Pregnant mice were intraperitoneally injected with LPS (300 μg/kg), Poly(I:C) (20 mg/kg), or NAC (300 mg/kg) at E16.5. The hearts of the newborn offspring mice were extracted within 12 h of birth [postnatal day (P)0] for mRNA sequencing and bioinformatic analysis. For hiPSC differentiation into cardiomyocytes, LPS (5 μg/mL), Poly(I:C) (10 μg/mL), or NAC (10 μM) was applied from day 16 to day 20 of differentiation. On day 20 of differentiation, cells were used for experiments. (A) Representative images of heart tissue stained with MitoSox Red/DAPI in each group (bar = 10 μm). (B) ATP content in offspring mouse heart tissue in each group. (C) The Ts2 to nuclear B2M DNA ratio in offspring mouse heart tissue in each group. (D) Representative images of hiPSC-B1-CM and hiPSC-F1-CM cell lines stained with Mitotracker/TNNT2/DAPI (bar = 20 μm). (E) The fold change of mRNA expression levels of metabolic pathway-related genes in hiPSC-B1-CM and hiPSC-F1-CM cell lines with or without LPS or Poly(I:C) challenge. (F) ATP content in hiPSC-B1-CM and hiPSC-F1-CM cell lines in each group. (G) Ts2 to nuclear B2M DNA ratio in hiPSC-B1-CM and hiPSC-F1-CM cell lines in each group. One-way analysis of variance (ANOVA) followed by Holm-Sidak post-test for multiple comparisons was used for comparing more than two groups (∗P < 0.05). The data were presented as mean ± standard error.
Credit
Yingrui Li, Willfredius Mugishagwe Rutahoile, Binquan Xiong, Jianlin Du, Songbai Deng, Bin Liu, Xiaodong Jing, Huiping Yang, Yue Han, Qiang She
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