Consumption of dietary β-fructan fibres in EAE mice is associated with altered immune cell populations in the caecum. (IMAGE)
Caption
EAE mice were fed β-fructan diet (n=3) or control fibre diet (n=3) beginning at symptom onset (day 14) until 23 days. At endpoint cecum tissues were collected for (A) single cell sequencing cell profiles, (B) quantitative flow cytometry of immune cell populations and (C) single-cell RNA sequencing–based differential gene expression and molecular function enrichment analyses. DC, dendritic cell; EAE, experimental autoimmune encephalomyelitis; MF, molecular function; mRNA, messenger RNA; NK, natural killer; pDC, plasmacytoid dendritic cell; rRNA, ribosomal RNA; Th1, T helper cell 1; Treg, regulatory T cell; UMAP, Uniform Manifold Approximation and Projection.
Credit
By Stephanie L Tollenaar, Reihane Khorasaniha, Heather Armstrong et al.
Usage Restrictions
Credit must be given to the creator. Only noncommercial uses of the work are permitted.
License
CC BY-NC