Generation of gene-edited liver organoids and multilineage liver organoids from primary cells, iPSCs/ESCs. (IMAGE)

First Hospital of Jilin University

Generation of gene-edited liver organoids and multilineage liver organoids from primary cells, iPSCs/ESCs.

Caption

Multicellular liver organoids can be generated using two main approaches. Primary liver cells can be directly co-cultured and self-organised into organoids, which can subsequently undergo gene editing to create genetically modified organoids. Alternatively, iPSCs or ESCs can be genetically edited at the pluripotent stage, differentiated into hepatocyte-like cells and various NPC types, and then assembled into multilineage, gene-edited liver organoids. Created with biorender.com. ESC, embryonic stem cell; iPSC, induced pluripotent stem cell; NPCs, non-parenchymal cells.

Credit

By Qi Rao, Lei Wang, Frank Tacke, Adrien Guillot, Nan Ma

Usage Restrictions

Credit must be given to the creator. Only noncommercial uses of the work are permitted.

License

CC BY-NC

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