Cellular ATP concentration, glucose metabolism, and oxidative stress. (IMAGE)
Caption
(A) ATP concentration of primary cardiomyocytes 72 h after transfection of virus (n = 3). (B) Glucose concentration in cell culture medium, 72 (Control, NC-AV, n = 4, cTnIR193H-AV, n = 3), 96 (n = 5), and 108 (n = 4) h after transfection of virus. (C) GLUT4 protein levels. (D) Statistical chart of GLUT4 protein expression levels (n = 4). (E) PI3K protein levels. (F) Statistical chart of PI3K protein expression levels (n = 5). (G) AKT and p-AKT protein levels. (H) Statistical chart of p-AKT/Akt protein expression ratio (n = 4). (I) PI3K protein levels. (J) Statistical chart of PI3K protein expression levels (n = 5). (K) AKT and p-AKT protein levels. (L) Statistical chart of p-AKT/Akt protein expression ratio (n = 4). (M) Glucose concentration in cell culture medium, 72 (n = 6), 96 (n = 6), and 108 (n = 5) h after transfection of virus. (N) Malondialdehyde (MDA) concentration (n = 4) and superoxide dismutase (SOD) activity (n = 3) in primary cardiomyocytes 130 h after transfection of virus. ∗P < 0.05 versus control; ∗∗∗P < 0.01 versus control; #P < 0.05 versus NC-AV; ##P < 0.01, ###P < 0.01, and ####P < 0.01 versus NC-AV; &P < 0.05 versus cTnIR193H-AV; &&P < 0.01, &&&P < 0.01, and &&&&P < 0.01 versus cTnIR193H-AV.
Credit
Min Luo, Lingjuan Liu, Wenjing Yuan, Junjun Quan, Mi Li, Jie Tian
Usage Restrictions
Credit must be given to the creator. Only noncommercial uses of the work are permitted. No derivatives or adaptations of the work are permitted.
License
CC BY-NC-ND