RBM47 serves as a target molecule for LINC00862. (IMAGE)
Caption
(A, B) Volcano plots of (A) RNA sequencing and (B) quantitative proteomics data are presented, with the criteria of fold change >2 or <0.5 and Q < 0.05 denoting up-regulation (red) or down-regulation (blue). The top 100 up- or down-regulated genes with respect to fold change are highlighted in a black frame. (C) The intersection of the top 100 up- or down-regulated RNAs and proteins was analyzed to identify the target gene. (D) The expression of RBM47 was measured by quantitative reverse transcription PCR (qRT-PCR) in both hepatocellular carcinoma (HCC) and paracancerous tissues (n = 84 for both). (E) A bivariate correlation analysis was performed to assess the relationship between LINC00862 and RBM47 in 84 HCC tissues. (F) Immunohistochemistry imaging was used to visualize RBM47 expression in HCC tissues with both high and low expression levels of LINC00862. Black bar = 100 μm. (G) Immunohistochemistry imaging was used to visualize the expression of RBM47 in xenografts derived from an empty control group and a LINC00862 overexpression group. Black bar = 100 μm. (H) qRT-PCR was employed to assess the levels of RBM47 mRNA in 5 hepatoma cell lines. (I) A bivariate correlation analysis was conducted to validate the association between LINC00862 and RBM47 mRNA in 5 hepatoma cell lines. (J) qRT-PCR and (K) western blotting were conducted to measure the expression levels of RBM47 in Huh7 cells (in which LINC00862 was silenced) and HCCLM3 cells (in which LINC00862 was overexpressed). ∗P < 0.05 and ∗∗∗P < 0.001.
Credit
Tao Guo, Yingying Jiang, Shunshun Zhu, Min Shi, Linying Sun, Juan Feng, Zhen Li, Cheng Gong
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