CHD5 participates in the transcriptional regulation of LINC00862 on RBM47. (IMAGE)
Caption
(A) A Venn diagram is used to elucidate the screening process of target proteins that co-interact with the RBM47 promoter and LINC00862. (B) Following the transfection of siRNAs in HCCLM3 cells, quantitative reverse transcription PCR (qRT-PCR) was employed to assess the knockdown efficiency of CHD5. The si-CHD5-1 was chosen for subsequent experimental investigations. (C, D) The expression of RBM47 is detected through (C) qRT-PCR and (D) western blotting after CHD5 silencing and LINC00862 overexpression. (E) Electrophoresis and qRT-PCR were utilized to evaluate the enrichment of LINC00862 normalized to IgG. (F) The CHD5 band was detected through western blotting after the RNA pulldown of the LINC00862 probe. (G) Electrophoresis and qRT-PCR were used to evaluate the enrichment of the RBM47 promoter after chromatin immunoprecipitation. (H) Western blotting was performed to detect CHD5 pulled by the RBM47 promoter via DNA pulldown assay. (I, J) qRT-PCR (I) and western blotting (J) were utilized to detect the enrichment of RBM47 promoter and CHD5, respectively, after chromatin isolation by RNA purification. NC, negative control. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.
Credit
Tao Guo, Yingying Jiang, Shunshun Zhu, Min Shi, Linying Sun, Juan Feng, Zhen Li, Cheng Gong
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