miR-378 was involved in TGFβ impaired osteogenesis in osteoporotic mice. (IMAGE)
Caption
A. ALP staining and Alizarin Red S staining of BMSCs from WT and miR-378 Tg mice upon sham and OVX operations, respectively and osteogenic induced for 7 days. Scale bar: 10 mm. B&C. Quantitative analysis of ALP activities (B) and ARS staining intensities (C). D-F. The mRNA expression level of osteogenesis markers including Runx2 (D), Ocn (E) and Opn (F) measured by real-time PCR. G-I. Total TNF〈 (G), IFN© (H) and TGFβ (I) serum levels in WT and miR-378 Tg mice after sham and OVX surgery. J. ALP staining and Alizarin Red S staining of BMSCs from WT and miR-378 Tg mice pretreated with TGFβ. Scale bar: 10 mm. K&L. Quantitative analysis of ALP activities (K) and ARS staining intensities (L). M-O. The mRNA expression level of osteogenesis markers including Runx2 (M), Ocn (N) and Opn (O) measured by real-time PCR (n=6; *p<0.05, **p<0.01, ***p<0.001).
Credit
Lu Feng, Zhengmeng Yang, Nan Hou, Haixing Wang, Shanshan Bai, Xuan Lu, Yaofeng Wang, Sien Lin, Micky D. Tortorella, Gang Li
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