CasRx-mediated knockdown of Ctnnb1 and Smo prevented OA knee joint degeneration. (IMAGE)
Caption
(A) Construction of AAV5 package plasmid. AAV5 delivered CasRx and S9C7 into the knee joint of DMM-induced OA mice. (B) Representative histology images of sham and osteoarthritic knee joints collected 3 months after injections of AAV5. Scale bar, 100 μm. n = 6–11. (C) Representative μCT images of sham and osteoarthritic knee joints collected 3 months after injections of AAV5. Red arrowheads, osteophytes. Scale bar, 2 mm. n = 3 or 4. (D) Representative Col2a1 immunohistochemistry images of sham and osteoarthritic knee joints collected 3 months after injections of AAV5. Scale bar, 100 μm. n = 6–11. (E–H) OARSI score (E), synovitis score (F), osteophyte size (G), and osteophyte maturity (H) of sham and osteoarthritic knee joints of the mice receiving AAVs. n = 6–11; one-way ANOVA followed by the Newman–Keuls test. (I) The damaged articular cartilage areas of knee joints were quantified by tracing the loss of safranin-O positive staining areas using the Image J system. n = 6–11; one-way ANOVA followed by the Newman–Keuls test. (J) Osteophyte volume of knee joints was quantified by μCT analysis in sham and osteoarthritic mice. n = 3 or 4; one-way ANOVA followed by the Newman–Keuls test. (K) Positive areas of Col2a1 of knee joints were quantified in sham and osteoarthritic mice. n = 6 or 7; one-way ANOVA followed by the Newman–Keuls test. Data are represented as mean ± standard error of the mean; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
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Genes & Diseases
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