CasRx-mediated gene knockdown decreased OA-associated factors in tissues. (IMAGE)
Caption
(A, B) Representative Smo/Ctnnb1 (A) and Adamts5/MMP13 (B) immunohistochemistry images of sham and osteoarthritic knee joints collected 3 months after injections of AAV5. Red arrowheads, immunohistochemistry-positive cells. Scale bar, 100 μm. n = 3–5. (C–F) Percentage of Smo (C), Ctnnb1 (D), Adamts5 (E), and MMP13 (F) positive cells were quantified in knee joints of sham and osteoarthritic mice. n = 3–5; one-way ANOVA followed by the Newman–Keuls test. (G, H) Representative immunofluorescence images showed CGRP expression (G) and average fluorescence intensity of CGRP (H) in L4 dorsal root ganglia of sham and DMM mice collected 3 months after injections of AAV5. Scale bar, 100 μm. n = 3; one-way ANOVA followed by the Newman–Keuls test. Data are represented as mean ± standard error of the mean; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
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Genes & Diseases
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