Efficient knockdown of the Ctnnb1 or Smo transcripts using CasRx in vitro (IMAGE)
Caption
(A) Design of 10 crRNAs matching Ctnnb1 or Smo transcripts. (B) Schematic of Ctnnb1 or Smo knockdown by CRISPR/CasRx. The sequences of the crRNA spacer are complementary to the Ctnnb1 or Smo transcripts (bold red line). (C) Schematic of plasmids used for crRNA screening (upper) and crRNA combination (lower) of Ctnnb1 or Smo knockdown in NIH 3T3 cells. Green cells indicated successful transfection of CasRx plasmids. (D) Knockdown of Ctnnb1 by different crRNAs (C1 and C2) in NIH 3T3 cells. n = 3; one-way ANOVA followed by the Dunnett test. (E) Knockdown of Smo by different crRNAs (S1–S10) in NIH 3T3 cells. n = 3; one-way ANOVA followed by the Dunnett test. (F) Knockdown of Ctnnb1 by combining C3 and C7 in NIH 3T3 cells. n = 3; unpaired student's t-test. (G) Knockdown of Smo by combining S9 and S10 in NIH 3T3 cells. n = 3; unpaired student's t-test. Data are represented as mean ± standard error of the mean; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
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Genes & Diseases
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