SLC3A1 mediated mitochondrial functions by NAD + uptake. (IMAGE)
Caption
SLC3A1 mediated mitochondrial functions by NAD + uptake. (A) Schematic diagram of SLC3A1 overexpression experiments. (B) Transcript expression of GSH metabolism related genes Gclc and Gclm in the transfected or non-transfected cells. (C) Transcript expression of fatty acid metabolism related genes Cpt1a, Acox1, Cpt2, and Acox2 in the transfected or non-transfected cells. (D) Representative images of double staining of SLC3A1 and the mitochondrial marker ATP5A1 in HK2 cells. Scale bar, 10 μm. (E) NAD+ content of control and SLC3A1 overexpressed cells. (F) NAD+ content of mitochondria isolated from HEK 293T control cells and cells stably overexpressing (OE) SLC3A1 before and after a 20-min incubation with 1 mM NAD+. (G) Scheme of the experimental approach. The mice were intraperitoneally injected with NMN (500 mg/kg) and then euthanized after 14 days. (H) Serum blood urea nitrogen (BUN) of Slc3a1 KO mice from both sexes with NMN supplement. (I) Relative mRNA levels of fibrosis and inflammatory genes (Kim1, Vimentin, and Col3a1) in the kidneys of Slc3a1 KO mice from both sexes with NMN supplement. (J, K) Representative images of hematoxylin/eosin- and Sirius red-stained kidney sections of Slc3a1 KO mice from both sexes with NMN supplement. Scale bar, 100 μm. All data are represented as mean ± standard deviation of the mean. p values were calculated by t-test with the post hoc Tukey test. ∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, ∗∗p < 0.01, ∗p < 0.05.
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Genes & Diseases
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