Beyond the toxin: Investigating the role of the cpb2 gene in the survival of Clostridium perfringens

A pivotal study has uncovered the genetic nuances of the cpb2 gene in Clostridium perfringens, a bacterium responsible for intestinal infections. The research indicates that cpb2 may enhance the bacterium's persistence in swine, offering critical insights into host adaptation and disease management.

C. perfringens is a widespread bacterium found in the intestines of animals and humans, known for causing severe enteric diseases. It produces over 20 toxins, with the β-2 toxin (CPB2) being linked to intestinal infections. CPB2, first identified in piglets with necrotizing enterocolitis, has since been found in multiple species. However, there is limited understanding of its genomic context and role in host adaptation. Due to these knowledge gaps, further research on the cpb2 gene and its role in pathogen evolution is essential.

The research (doi: 10.1186/s44280-024-00058-8), conducted by scientists at Northwest A&F University, was published on August 30, 2024, in the journal One Health Advances. The study aimed to investigate the genomic characteristics of C. perfringens strains carrying the cpb2 gene, with a particular focus on the potential biological function of the gene and its host specificity.

The study involved the analysis of 763 C. perfringens genomes from various hosts, revealing a high prevalence of the cpb2 gene in pig-derived strains. Interestingly, while the cpb2 gene was enriched in pigs, it did not appear to affect the evolutionary relationships of the bacterial strains. Instead, the gene is located on a conserved genetic segment within the plasmids, indicating potential horizontal transfer between strains. The researchers also discovered that the gene is consistently located on a specific segment containing a transcriptional regulator, suggesting a mechanism for its persistence in certain hosts. Additionally, the research showed that the cpb2 gene was associated with host species-specific adaptations, particularly favoring the porcine intestinal environment. This finding suggests that C. perfringens may exploit this gene to better survive in pigs, although the gene's function in other animals remains less clear.

Dr. Juan Wang, the lead author of the study, emphasized the importance of these findings: "Our research provides a detailed genomic analysis of the cpb2 gene, and its consistent presence in pig-derived strains suggests a key role in host adaptation. Understanding how this gene functions could lead to new insights into preventing C. perfringens infections, particularly in the livestock industry."

The findings of this study have broad implications for public health and the livestock sector. Since C. perfringens is a major pathogen in pigs, understanding the genetic mechanisms behind its adaptation could inform new strategies for controlling infections. What’s more, these insights into the cpb2 gene could help in developing targeted interventions to prevent C. perfringens-related diseases in both animals and humans.

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References

DOI

10.1186/s44280-024-00058-8

Original Source URL

https://doi.org/10.1186/s44280-024-00058-8

Funding information

This work was supported by the Natural Science Basic Research Plan in Shaanxi Province of China (No. 2021-KW-41), the China Agriculture Research System of MOF and MARA (CARS-39) and Ganzi Prefecture Science and Technology Plan Project of 2022 (22kjjh0016).

About One Health Advances

One Health Advances is a peer-reviewed, open access journal committed to publish high-quality research articles, comments, correspondence and reviews describing the advances in public health. The topics primarily focus on antimicrobial resistance, animal-derived food safety, and animal diseases with a preference for zoonoses. Prof. Jianzhong Shen (China Agricultural University, China) and Prof. Stefan Schwarz (Freie Universität Berlin, Germany) serve as the Editors-in-Chief.