image: The breeding process of MAS for backcrossing to introduce translocation chromosome. (A) HMF was the backcross parent variety. The red text presented the heterozygous translocation line (Aa) and homozygous translocation line (AA) by MAS in each generation. (B) Heterozygous translocation lines (Aa) were screened on the BC1 population using the translocation recombination marker Chr10 + Chr106; Homozygous translocation lines (AA) were screened on the BC4F2 population. (C) Wild-type HMM and homozygous translocation line HMF-TL were used to prepare the hybrid combination “Less-seed HM F1”. Compared with “CK HM F1”, which used wild-type “HMM” and wild-type “HMF” as the paternal parent to prepare the hybrid group, the fruit transection section seed number ratio of “Less-seed HM F1” was significantly reduced.
Credit: Horticulture Research
In an agricultural advancement, scientists have deciphered the genetic underpinnings of watermelon chromosomal translocations, the key to cultivating fruits with fewer seeds.
The global demand for seedless watermelons has surged due to their appeal to consumers. Traditional methods, such as inducing triploidy, have led production but face significant challenges like low seed production, germination rates, and poor seedling establishment. Additionally, reliance on growth regulators raises environmental and health concerns. Based on these challenges, alternative genetic techniques are needed to enhance seedless watermelon breeding. Chromosomal translocation offers a promising solution, potentially overcoming these obstacles and paving the way for more efficient and sustainable production practices.
A team of researchers from the Chinese Academy of Agricultural Sciences, in collaboration with the Beijing Academy of Agriculture and Forestry Science, published their findings (DOI: 10.1093/hr/uhae087) on April 22, 2024, in Horticulture Research. The study focused on developing less-seed watermelon varieties through chromosomal translocation, utilizing advanced genome sequencing technologies and molecular markers.
The study analyzed three groups of chromosomal translocation materials from different sources to develop less-seed watermelons. Using third-generation genome sequencing and fluorescence in situ hybridization (FISH), researchers identified specific reciprocal translocations between chromosomes. For instance, naturally mutated material MT-a showed translocations between Chr6 and Chr10, while radiation-induced mutants MT-b and MT-c involved translocations between Chr1 and Chr5, Chr4 and Chr8, and a complex translocation involving Chr1, Chr5, and Chr11. These translocations significantly reduced seed counts in hybrid fruits. The researchers also developed molecular markers to efficiently identify these translocation lines. This approach provides a robust method for breeding less-seed watermelons without the use of growth regulators, offering a sustainable alternative to traditional seedless watermelon production methods and addressing key challenges in the industry.
Dr. Wenge Liu, the lead researcher, stated, "This breakthrough in understanding chromosomal translocations in watermelons opens new avenues for breeding less-seed varieties. The use of advanced sequencing technologies and molecular markers significantly enhances our ability to develop commercially viable seedless watermelons."
The findings of this study have significant implications for watermelon breeding. By utilizing chromosomal translocation techniques, breeders can overcome the limitations of traditional seedless watermelon production methods. This research not only paves the way for more efficient breeding practices but also has the potential to enhance the quality and yield of watermelons, meeting consumer demand and supporting agricultural innovation.
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References
DOI
Original Source URL
https://doi.org/10.1093/hr/uhae087
Funding information
This work was financially supported by the National Natural Science Foundation of China (Grant No. 32330093), the Ministry of Agriculture and Rural Affairs of China (CARS-25), the Scientist Training Program of BAAFS (JKZX202401), the Collaborative Innovation Center of BAAFS (KJCX201907-2), Innovation and Development Program of BVRC (KYCX202401), the National Key Research and Development Program of China (2023YFF1000100), agricultural Science, and Technology Innovation Program (CAAS-ASTIP-2021-ZFRI).
About Horticulture Research
Horticulture Research is an open access journal of Nanjing Agricultural University and ranked number one in the Horticulture category of the Journal Citation Reports ™ from Clarivate, 2022. The journal is committed to publishing original research articles, reviews, perspectives, comments, correspondence articles and letters to the editor related to all major horticultural plants and disciplines, including biotechnology, breeding, cellular and molecular biology, evolution, genetics, inter-species interactions, physiology, and the origination and domestication of crops.
Journal
Horticulture Research
Subject of Research
Not applicable
Article Title
Identification of allelic relationship and translocation region among chromosomal translocation lines that leads to less-seed watermelon
Article Publication Date
22-Apr-2024
COI Statement
The authors declare that they have no competing interests.