Cellular communication analysis identifies DLL3 as a pivotal hub bridging malignant progression and immunosuppression. (IMAGE)

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Cellular communication analysis identifies DLL3 as a pivotal hub bridging malignant progression and immunosuppression.

Caption

:  (A) DLL3-NOTCH1/2 and CD38-PECAM1 interactions expressed in NECC and ADC/SCC samples. The cell subgroups and ligand–receptor interactions are indicated by the rows and columns, respectively. The means of the average expression levels of the two interacting molecules are indicated by the color key, with white to red representing low to high expression. The [−Log10(p-values)] are indicated by dot size. (B) Correlation between 2-gene signature (CD38, PECAM1) and 14-gene adenosine signature (PPARG, CYBB, COL3A1, FOXP3, LAG3, APP, CD81, GPI, PTGS2, CASP1, FOS, MAPK1, MAPK3, CREB1) or 7-gene immune checkpoint signatures (HAVCR2, TIGIT, LAG3, PDCD1, LAYN, CTLA4, TIM3) in CC at TCGA database. (C) Schematic diagram showing cellular communications (CD38-PECAM1, DLL3-NOTCH1/2) between NPCs and TILs in NECC. (D) A UMAP plot showing the expression of DLL3 of NECC. The color keys, ranging from gray to red, indicate relative expression levels from low to high. (E) The differences in immunohistochemical scores of DLL3 among 14 NECC, 9 ADC, and 7 SCC samples through IHC analysis. (F) DLL3 expression in representative NECC, ADC, and SCC samples by IHC staining. scale bars, 200 μm and 50 μm for the top and bottom panels, respectively. Abbreviations: NECC, neuroendocrine carcinoma of the cervix; TIME, tumor immune microenvironment; ADC, adenocarcinomas of the cervix; SCC, squamous cell carcinomas; TCGA, The Cancer Genome Atlas; NPCs, neuronal progenitor cells; TILs, tumor infiltrating lymphocytes; ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001. ns, not significant; Error bar: mean value with 95% CI.

Credit

Qinqin Liu, Xinyu Qu, Shuqi Li, Yan Ding, Tingting Ren, Qi'an Jiang, Jingxin Ding, Keqin Hua, Junjun Qiu

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