PCBP2 is a mediating RBP for circLPAR3 and CHAC1. (IMAGE)
Caption
(A) Silver staining assay and Western blotting assay were conducted to verify the binding of circLPAR3 to PCBP2. (B) PCBP2-RNA immunoprecipitation assay was conducted to verify the binding of PCBP2 to circLPAR3. (C) Fluorescence in situ hybridization and immunofluorescence assays were carried out to indicate the co-localization of circLPAR3 and PCBP2. (D) PCBP2-RNA immunoprecipitation assay was conducted to verify the binding of PCBP2 to CHAC1. (E) Binding of PCBP2 on CHAC1 mRNA was predicted using RBPsuite Predicted. ∗∗∗P < 0.001 and ∗∗∗∗P < 0.0001.
Credit
Yetao Zhang, Yuxiang Dong, Kai Li, Tong Zhao, Yongshan Li, Mingyang Pang, Yong Wei, Bing Yao, Qingyi Zhu
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