Functional characterization of genes during self-incompatibility response. (IMAGE)
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(A–D) Representative aniline blue images of pollinated stigmas treated with Mock, Sense, and Anti-Sense ODNs of (A)SRK, (B)FER1, (C)MLPK, and (D)ARC1, showing pollen attachment and tube formation. Note: Aniline blue (first), Red channel (second), Bright field (third), Zoomed (fourth). Note: Scale bars: 300 μm & 50 μm (zoomed), white arrows indicate pollen grains with emerging pollen tubes.
(E–H) Graph representing the number of pollen grains per stigma observed in (Figures 5A–D); treated with Mock, Sense, and Anti-Sense ODNs of (E) SRK, (F) FER1, (G) MLPK, and (H) ARC1. Note: Dots represent individual data points. One way ANOVA, *P < 0.05, n = 9, ns, not significant.
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Please credit the Indian Institute of Technology Gandhinagar (IITGN) for this research.
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