Simultaneous observation of two fluorescent proteins within plant cells (IMAGE)

Institute of Transformative Bio-Molecules (ITbM), Nagoya University

Simultaneous observation of two fluorescent proteins within plant cells

Caption

Simultaneous observation of two fluorescent proteins within plant cells. Panels (A, B) show same-colored proteins are indistinguishable by conventional fluorescence intensity imaging. Panels (C–F) are Phasor Plot analyses that successfully distinguished between same-colored proteins. Upper panels (A, C, E) show experiments with red fluorescent proteins (mCherry and tagRFP-T), while lower panels (B, D, F) show experiments with green fluorescent proteins (NowGFP and GFP). These results demonstrate that FLIM data is effective regardless of the color emission.

Credit

WPI-ITbM

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