CUDC-907 regulates the cell cycle of glioblastoma cells by inhibiting MYC expression to suppress glioblastoma cell proliferation. (IMAGE)
Caption
(A) RNA sequencing analysis was conducted on LN229 cells exposed to 10 nM CUDC-907 or DMSO for 24 h. The KEGG enrichment analysis of total differential genes. (B) The cell cycle was examined using Gene Set Enrichment Analysis (GSEA) based on the differentially expressed genes following CUDC-907 treatment in LN229 cells. The P value was calculated with the GSEA software. (C) Cell cycles of both cell lines were arrested in the G1 phase after 48 h of treatment with CUDC-907. (D) The levels of CDK2, CDK4, p21, and MYC were assessed by Western blotting in LN229 and A172 following 48 h of CUDC-907 exposure. DMSO was used as a control. (E) EdU-positive LN229 and A172 cells were identified following treatment with CUDC-907 (10 nM) or DMSO for 48 h. Scale bars = 50 μm. (F) The impact of indicated concentrations of CUDC-907 or DMSO on the colony formation capabilities of LN229 and A172. The data were expressed as mean ± standard deviation. Student’s t-test was performed to analyze significance. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.
Credit
Chencheng Fang, Pan Gou, Dandan Zhang, Xuanxuan Wu, Xiao Li, Man Li, Lu Gan, Jinjin Luo, Hongjuan Cui, Man Xu, Ping Liang
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