Up-regulation of AC004854.2 in HCT116 and HT-29 cells enhances proliferation, suppresses apoptosis and G1-phase arrest, and promotes invasion and metastasis. (IMAGE)

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Up-regulation of AC004854.2 in HCT116 and HT-29 cells enhances proliferation, suppresses apoptosis and G1-phase arrest, and promotes invasion and metastasis.

Caption

(A) Quantitative reverse transcription PCR confirmed efficient overexpression of AC004854.2 in HCT116 and HT-29 cells. (B–F) Upon AC004854.2 up-regulation. (B) CCK-8 assays showed that increased cell viability was observed. (C) BrdU incorporation assays showed elevated DNA synthesis. (D) Colony formation capacity was significantly enhanced. (E, F) Flow cytometry revealed reduced apoptotic cell populations (E) and a shift in cell cycle distribution consistent with decreased G1-phase arrest (F). (G–I) Functional assays demonstrated increased migratory and invasive potential. (G) Wound healing assays showed accelerated wound closure. (H) Transwell migration assays revealed greater cell motility. (I) Matrigel-based invasion assays indicated enhanced invasive capability. Statistical analyses: Wilcoxon rank-sum test, Student's t-test; significance levels: ns, not significant; ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.

Credit

Yanjie Chen, Rui Wang, Dexiang Zhu, Lisha Cheng, Ji Zhao, Xiguo, Li Liang, Tianshu Liu

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