ROLLCSC acts as a ceRNA to promote SLC25A11. (IMAGE)

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ROLLCSC acts as a ceRNA to promote SLC25A11.

Caption

(A) Schematic diagram of the construction of ceRNA of ROLLCSC network. (B) The target genes of miR-5623-3p were enriched by KEGG and GO analysis; Only partial data of lipid-related pathways are shown. (C) The target genes of miR-5623-3p were combined with TCGA-LUAD data to perform GSEA analysis (Sangerbox; http://vip.sangerbox.com/home.html). (D) Multiple datasets further predict target genes of miR-5623-3p, visualized by Venn diagram. (E) Survival analysis of target gene (D) in TCGA-LUAD data and visualized by Sangerbox. (F) PCR detected the regulatory effect of miR-5623-3p on Gss and Slc25a11.(G) PCR detected the regulatory effect of ROLLCSC on Slc25a11. (H) The wild-type and mutant-type plasmids of Slc25a11 in Dual-luciferase. (I) Dual luciferase assay detected the direct binding of Slc25a11 to miR-5623-3p. (J) Effect of ROLLCSC on GSH in mitochondria and cytoplasm of LLC cells. (K) Effect of Slc25a11 on GSH in mitochondria and cytoplasm of LLC-oeROLLCSC cells. (L) Effect of ROLLCSC and Slc25a11 on mito-ROS (red fluorescence) in LLC cells. (M) Representative images and statistical analysis of transwell results suggest the effect of Acsl4 overexpression on the migration (upper) of LLC cells. Six randomly selected images per group were used for data analysis. (N) Representative images and statistical analysis of transwell results suggest the effect of Acsl4 overexpression on the migration (upper) of LLC cells. Six randomly selected images per group were used for data analysis. (O) Lung metastatic lesions were assessed by fluorescence imaging and H&E staining after euthanasia. Images are representative of three independent mice (n = 3). (P) Effect of Slc25a11 on ROS and mito-GSH in lung metastases from C57BL/6 mice, number of samples (n = 3). (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. ns, not significant. The results represent three independent experiments.)

Credit

Yu-Han Zhang, Jia-Cheng Xie, Ting Ye, Shi-Meng Guo, Xue Han, Si Yang, Lei Shi, Yi-Shi Li, H. Rosie Xing, Jing-Yu Li, Jian-Yu Wang

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