NRG4 facilitates the interaction between pERBB4 and pYAP1 to hinder the nuclear translocation of YAP1 (IMAGE)
Caption
(A) Co-IP was used to enrich for ERBB4-bound proteins, followed by silver staining of differential bands and protein mass spectrometry for identifying 162 binding proteins in 4T1 cells. (B) The number of ERBB4 binding proteins in LC/MS assays upon rNRG4 treatment for 24 h. (C) STRING analysis revealed the interaction between ERBB4 and YAP1. (D) Western blotting analysis after nuclear-cytoplasmic fractionation revealed increased pERBB4 and pYAP1 in the cytoplasm and reduced YAP1 expression in the nucleus after rNRG4 treatment. (E) Co-IP of ERBB4 in the cytoplasm of 4T1 cells treated with rNRG4, followed by immunoblotting. (F) qPCR analysis of the mRNA expression levels of EMT-related genes in MDA-MB-231 cells overexpressing YAP5SA in the presence of NRG4. (G) qPCR analysis of the expression levels of Tead subunits in 4T1 cells and MDA-MB-231 cells. (H) qPCR analysis of the mRNA expression levels of Tead1 in 4T1 cells and MDA-MB-231 cells treated with 100 ng/mL rNRG4. (I) Co-IP results demonstrated the decrease of TEAD1 and YAP1 binding by rNRG4 treatment. The data were presented as mean ± standard deviation. ∗p < 0.05 and ∗∗p < 0.01.
Credit
Saijun Wang, Mingwei Guo, Lingyun Xu, Jiaming Xue, Shuai Chen, Ke Xu, Yan Zhou, Aihua Gu, Wei Gao, Jianwei Zhou, Yi Zhang, Liming Tang, Dongmei Wang
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