FOXJ3 is expressed in NSCs in the VZ and neurons in the CP during cortical development. (IMAGE)
Caption
(A) Western blot analysis of FOXJ3 protein expression in the developing mouse cortex from E11.5 to E18.5. FOXJ3 expression was detected at all examined stages and increased progressively. (B) Bar graph showing relative FOXJ3 protein expression across developmental stages. Error bars represent S.E.M. (n = 3 independent experiments). (C) Immunostaining of cortical cryosections reveals FOXJ3 expression (gray, red) in the VZ and CP from E12.5 to E18.5. FOXJ3 levels in the VZ was high before E14.5 and declined sharply after E15.5. Sparse expression of FOXJ3 was observed in the IZ, while CP expression persisted. Brain slices were counterstained with DAPI (blue) to show cell nuclei. Scale bars: 50 µm (top panels), 10 µm (lower panels). (D) Bar graph showing normalized FOXJ3 fluorescence intensity in VZ. Error
bars represent S.E.M. **: p < 0.01, ****: p < 0.0001 (n = 3 independent experiments; one-way ANOVA with post-hoc Dunnett test; p = 0.0025, p < 0.0001, 0.0001, 0.0001). (E) Primary culture cortical neurons from E14 mouse embryos were infected with GFP (green) and immunostained with anti-FOXJ3 antibody (magenta) and DAPI (blue). Scale bar: 10 µm.
Credit
the authors
Usage Restrictions
Credit must be given to the creator. Only noncommercial uses of the work are permitted. No derivatives or adaptations of the work are permitted.
License
CC BY-NC-ND