FASN's contribution to PARPi resistance. (IMAGE)

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FASN's contribution to PARPi resistance.

Caption

(A, B) Western blotting analysis of FASN and PARP1 expression in stable MDA-MB-436 cells overexpressing FASN (MDA-MB-436/FASN) and MDA-MB-231 cells with FASN knockdown (MDA-MB-231/shFASN) along with their respective control cells transfected with vector (Vec) and scrambled ShRNA (ShFASN). 

(C–F) Concentration-dependent survival curves of MDA-MB-436/FASN cells, MDA-MB-231/shFASN cells, and their respective control cells. Their relative resistance factor (RRF) against talazoparib and 5HLS was assessed using the methylene blue survival assay to derive IC50 values and calculated using the formula RRF = IC50(FASN or shFASN)/IC50(Vec or Scr). The data are presented as mean ± standard deviation. n = 3; ∗∗P < 0.01 and ∗P < 0.05.

Credit

Sophia Josephraj, Chao J. Wang, Qingbin Cui, Zizheng Dong, Jing-Yuan Liu, Jian-Ting Zhang

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