The summary of DNA nucleases. (IMAGE)
Caption
A, B) Zinc finger nucleases (ZFNs) and transcription activator-like effect nucleases (TALENs) edit genome by programmable protein binding to targeting site.
(C) Clustered regularly interspaced short palindromic repeats associated protein 9 (CRISPR-Cas9) edit genome by programmable RNA:DNA interaction.
(D) ZFNs, TALENs, and CRISPR-Cas9 create double-strand DNA breaks (DSBs) initiating DNA-damage repair and defense response mediating by functional TP53 and KRAS, and increasing the risk of chromosome loss or truncation.
(E, F) Base editors (BEs) and prime editors (PEs) edit genome DSBs cutting one strand of DNA, without creating DSB.
Credit
Tao Wang, Mingyang Yu, Ping Liu, Zhiqiang Song, Cheng Li, Jianmin Yang, Na Liu
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