circFKBP8(5S,6) or its encoded protein cFKBP8 down-regulates the expression level of DRD3 and its downstream AMPK/mTOR/ULK1 signaling pathway in PrL of CUMS mice. (IMAGE)
Caption
(A) The Venn diagram showed the distribution of the DEGs between circFKBP8(5S,6) and cFKBP8-regulated genes. (B, C) DEGs involved in the neuroactive ligand-receptor interaction pathway are co-regulated by cFKBP8 (B) and circFKBP8(5S,6) (C). (D) The mRNA levels of DRD3 after overexpression of circFKBP8(5S,6) or overexpression of cFKBP8 in the PrL of mice. n = 6. (E, F) Western blotting (E) and quantitation (F) for the level of DRD3 in the PrL of mice. n = 6. (G) Representative immunofluorescence staining images of DRD3 (DRD3-labeling, red) in PrL neurons (EGFP-labeling, green). Scale bar = 20 μm. (H) Quantification of the red fluorescence intensity of labeled DRD3 co-labeled with EGFP. n = 3. (I–N) Representative western blots (I) and quantitative evaluations (J–N) of the cFKBP8, p-AMPK/AMPK, p-mTOR/mTOR, p-ULK1 (Ser555)/ULK1, p-ULK1 (Ser757)/ULK1, and p-ULK1 (Ser317)/ULK1 in the PrL of mice exposed to different treatments. n = 6. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001; ns, non-significant. Data were represented as mean ± standard error of the mean. DRD3, dopamine D3 receptor; CUMS, chronic unpredictable mild stress; DEGs, differentially expressed genes; PrL, prelimbic cortex; AMPK, adenosine 5′-monophosphate-activated protein kinase; mTOR, mammalian target of rapamycin; ULK1, unc-51-like kinase 1; EGFP, enhanced green fluorescent protein.
Credit
Dandan Xu, Zihan Huang, Gaojia Zhang, Jiao Jiao, Yujia Cao, Mengyu Liu, Yan Kong, Zhijun Zhang
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