LVNC-hiPSC-CMs have abnormal skeleton, polarity, and mitochondria compared with the NC-hiPSC-CMs. (IMAGE)

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LVNC-hiPSC-CMs have abnormal skeleton, polarity, and mitochondria compared with the NC-hiPSC-CMs.

Caption

Representative transmission electron microscope images of hiPSC-CMs between the NC group and LVNC group. Myofibrils, yellow arrow; endoplasmic reticulum, blue arrow; mitochondria, green arrow. (B, C) The analysis of the proportion of the vacuolated mitochondria (n = 3 samples per group) and ATP content (nmol per mg protein) in hiPSC-CMs among the NC-hiPSC-CMs and LVNC-hiPSC-CMs groups (n = 3 samples per group). (D–H) Immunostaining of hiPSC-CMs for F-actin, vinculin, and Par3, and the analysis of the fluorescence intensity level (n = 30–80 cells per group). ∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, and ∗p < 0.05 versus the NC group.

Credit

Wuxia Gu, Hongyan Li, Wenjing Yuan, Xiaoqiong Fu, Rui Wang, Xiaohui Xu, Xuemei Liao, LingJuan Liu, Bo Pan, Jie Tian, Haixin Yuan, Yi Huang, Tiewei Lu

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