[Figure 2] Detection of 2.5 copies of 4 doping genes and single cell-level of genetically modified cellss using CRISPR (IMAGE)

National Research Council of Science & Technology

[Figure 2] Detection of 2.5 copies of 4 doping genes and single cell-level of genetically modified cellss using CRISPR

Caption

Very trace amounts of doping genes and transgenic cell models spiked in blood are amplified directly without nucleic acid extraction, and the presence or absence of targets is then confirmed by sequence-dependent fluorescence reaction of Cas12a, a type of gene scissors. The entire analysis time from blood to readout is as short as 90 minutes.

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Korea Institute of Science and Technology(KIST)

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