AP-4 complex mediates the sorting of VAMP727 for vacuolar transport at the trans-Golgi network (IMAGE)
Caption
Super-resolution confocal live imaging microscopy (SCLIM) reveals the subcellular localization of VAMP727 in wild-type and ap4m mutant Arabidopsis root epidermal cells. In wild-type cells (A and B), VAMP727 predominantly localizes to late endosomes, the trans-Golgi network (TGN), and the interface between these compartments. In ap4m mutants lacking a functional AP-4 complex, VAMP727 exhibits a broadened distribution across the TGN and diminished presence in late endosomes (B). These observations indicate that the AP-4 complex plays a critical role in partitioning VAMP727 within the TGN to facilitate its selective incorporation into the vacuolar transport pathway. Scale bars: 1 µm.
Credit
Yutaro Shimizu and Masaru Fujimoto
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