AlphaFold3-Predicted Substrate Interactions and Catalytic Site Conservation of Amuc_1547. (IMAGE)

Sichuan International Medical Exchange and Promotion Association

AlphaFold3-Predicted Substrate Interactions and Catalytic Site Conservation of Amuc_1547.

Caption

(a) Interaction of key residues involved in substrate binding in the AlphaFold3 predicted complex of Amuc_1547 with substrate SIA (d) and 6'SL (e). 6'SL (6'-Sialyllactose): A glycoside compound composed of Sialic acid (SIA), Galactose (GAL), and N-Acetylglucosamine (NAG). (b) Structural comparison of catalytic centers of Amuc_1547 with other GH33 sialidases. RgNanH (PDB code: 4X47) in light green, TrSA (PDB code: 1N1Y) in light blue, BTSA (PDB code: 4BBW) in lilac, and Amuc_1547 in rose red. The GH33 family catalytic active center residues, including an arginine triplet (3 Arg), a pair of nucleophilic amino acids (Glu and Tyr), and an acid-base catalytic amino acid (Asp) were displayed in stick. (c) Kinetic analysis and parameters of Amuc_1547 enzymes and mutants. All mutant proteins (purity > 90%) were purified using the same protease method as the wild-type.

Credit

Dr. Tao Li and Dr. Rui Bao

Usage Restrictions

Please cite the source.

License

Original content

Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.