YTHDF1 in hematopoietic cells is necessary for the suppression of inflammatory response. (IMAGE)
Caption
(A–F) Eight weeks old male Ythdf1−/− mice (KO) were irradiated with 8 Gy X-rays, and 6 h later, bone marrow cells (BM) from male donor Ythdf1−/− or WT mice were transplanted into the irradiated mice with an intravenous injection at a number of 200E4 cells per mouse (A). Four weeks after transplantation, mice were intravenously injected with ConA (8 mg/kg) or saline for 8 h, n = 3–5 per group. Serum samples were measured for ALT (B), IL-6 (C), and CXCL1 (D) levels. Liver sections were analyzed by H&E staining (E) and the necrotic area was counted for statistical analysis (F). (G–L) Eight weeks old male WT mice were irradiated with 8 Gy X-rays, and 6 h later, bone marrow cells from male donor Ythdf1−/− or WT mice were transplanted into the irradiated mice with an intravenous injection at a number of 200E4 cells per mouse (G). Four weeks after transplantation, mice were intravenously injected with ConA (8 mg/kg) or saline for 8 h, n = 3–5 per group. Serum samples were measured for ALT (H), IL-6 (I), and CXCL1 (J) levels. Liver sections were analyzed by H&E staining (K) and the necrotic area was counted for statistical analysis (L). Data are presented as mean ± SD; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. BMT, bone marrow transplantation. KO, Ythdf1−/−.
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Genes & Diseases
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