The protein level of m6A reader YTHDF1, YTHDF2 and YTHDF3 decrease during the early stage of ConA-induced hepatitis. Eight-week-old male C57BL/6 WT mice were intravenously injected with ConA (10 mg/kg) or saline as vehicle control, and the liver tissues w (IMAGE)

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The protein level of m6A reader YTHDF1, YTHDF2 and YTHDF3 decrease during the early stage of ConA-induced hepatitis. Eight-week-old male C57BL/6 WT mice were intravenously injected with ConA (10 mg/kg) or saline as vehicle control, and the liver tissues w

Caption

(A, B) Immunoblot analysis of liver tissues with m6A family and representative inflammatory pathway antibodies (A), followed by gray analysis using Image J (B). (C) Representative images of liver sections for immunostaining analysis with YTHDF1, YTHDF2 and YTHDF3 antibodies, Scale bar = 100 μm. (D) Heatmap analysis from liver RNA-seq data indicated the mRNA expression level of the m6A family after ConA treatment for 8 h. (E, F) Heatmap analysis from the GEO database showed the fold change of m6A family mRNA levels after the ConA challenge at indicated time points compared with vehicle control in liver tissues. Data are presented as mean ± SD; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 compared to the control group of WT ConA 0h, based on unpaired two-sided Student's t-test.

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Genes & Diseases

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