Analysis of S1pr3 expression in the development of BLM-induced pulmonary fibrosis in mice. (IMAGE)
Caption
: (A) Representative images of S1pr3 immunofluorescence staining (upper panel, scale bar = 50 μm), HE staining (middle panel, scale bar = 100 μm), and Masson's trichrome staining (lower panel, scale bar = 25 μm) of lung tissues after BLM induction at different time points. The bar graph on the right shows quantitative S1pr3 expression and numerical fibrotic score (Ashcroft score). (B) Quantification of the hydroxyproline content of lung tissues at different time points after BLM administration. (C, D) Quantitative reverse transcription-PCR and Western blot analyses of S1pr3, fibronectin, and collagen I of lung homogenates. BLM, bleomycin; D0, normal control; D3/D7/D14, 3/7/14 days after BLM stimulation; MFI, mean fluorescence intensity; Col I, collagen I; Fib, fibronectin. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.
Credit
Genes & Diseases
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