Conditional deletion of S1pr3 in macrophages inhibited BLM-induced pulmonary fibrosis in mice. (IMAGE)
Caption
(A) Breeding strategy of LysM-Cre+/S1pr3flox/flox (S1pr3-CKO) and LysM-Cre−/S1pr3flox/flox (S1pr3-C) mice. (B) Representative images for co-immunostaining of CD115 and S1pr3 in lung sections from S1pr3-C and S1pr3-CKO mice on day 14 after BLM stimulation. Nuclei were stained with DAPI (blue); scale bar = 50 μm. (C) Representative images of HE staining (upper panel, scale bar = 100 μm) and Masson's trichrome staining (lower panel, scale bar = 25 μm) of lung tissues of S1pr3-C and S1pr3-CKO mice after BLM challenge. The bar graph shows the Ashcroft score. (D) Quantification of hydroxyproline content. (E, F) Quantitative reverse transcription-PCR and Western blot analysis of lung collagen I and fibronectin in S1pr3-C and S1pr3-CKO mice after BLM challenge. Col I, collagen I; Fib, fibronectin; DAPI, 4′,6-diamidino-2-phenylindole; ns, not significant. ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.
Credit
Genes & Diseases
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