Labeled DNA fibers used in this study (IMAGE)

National Institutes of Natural Sciences

Labeled DNA fibers used in this study

Caption

In the DNA fiber assay used in this study, cells labeled with IdU (green) and CldU (red) nucleotide analogs were sorted into substages of S phase before being subjected to the DNA fiber assay. This method allows for the measurement of various parameters, such as replication fork speed, fork pausing rate, and origin-firing rate in substages of S phase.

Credit

K. Kurashima

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