Hypomagnetic fields modulate lifespan, physical ability and mitochondrial metabolism in a Pink1 model of neurodegeneration (IMAGE)
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Figure 3. High resolution respirometry and mitochondrial free radical detection of control (WT) and Pink1- D. Melanogaster. (A, B) Mitochondrial oxygen flux across the SUIT protocol for (A) WT (GMF vs. 10D HMF) and (B) Pink1– (GMF vs. 10D HMF) flies, from a linear mixed-effects model (flux ~ group × state, replicate as a random intercept) with Tukey-adjusted pairwise comparisons; only significant comparisons (p<0.05) are annotated. Pink1- CI+CII OXPHOS values between GMF and 10D HMF were significantly different (p=0.035) (C, D) Complex I– and Complex II–linked OXPHOS capacity analysed as separate pairwise comparisons (Welch two-sample t-test) for (C) WT and (D) Pink1– flies, where CII OXPHOS = (CI+CII OXPHOS) − CI OXPHOS, calculated per replicate. CII OXPHOS was significantly elevated by 10D HMF treatment in WT flies (p=0.027) but not in Pink1– flies (p=0.275); CI OXPHOS did not differ significantly between treatments in either genotype. (E, F) TEMPOL-mediated Modulation of nitrogen vacancy centres in nanodiamonds via Magnetic Modulation (MM) contrast with homogenised WT and Pink1- D. melanogaster All Photoluminescence (PL) contrast values were normalised to a control only including TEMPOL and Mir05. (n = 5 replicates). (E) WT and Pink1- D. melanogaster showed an increased level of free radical formation under Routine and OXPHOS in HMF states. (F) The change between routine and OXPHOS was not changed in WT flies when comparing the two treatments, however in Pink1- D. melanogaster the fold change was markedly decreased in the HMF state and increased in the GMF state, when compared to WT results. n=5 per treatment group; two-tailed, multiple unpaired t-tests. Omnibus mixed model ANOVA there is a significant treatment specific effect (p = 0.0015 **) and respiratory state effect from Routine to OXPHOS (p<0.0001 ***) between all samples. Error bars show ±SEM.
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