Figure 1. AZD1390 protects against erastin-induced ferroptosis in MDA-MB-231 cells. (IMAGE)

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Figure 1. AZD1390 protects against erastin-induced ferroptosis in MDA-MB-231 cells.

Caption

(a) ATM inhibitors protect MDA-MB-231 cells against ferroptosis. MDA-MB-231 cells were treated with increasing doses of erastin (24 h), either alone or in combination with liproxstatin-1 (LPT1, 2 μM), KU55933 (5 μM), and KU60019 (1 μM). Cell viability was quantified by CellTiter-Glo assay; (b, c) AZD1390 reduced erastin-induced ferroptosis in MDA-MB-231 cells in a dose-dependent manner. After 24 hours of incubation, cell viability and cytotoxicity were determined by CellTiter-Glo assay (b) and CellTox Green assay (c), respectively. Canonical ferroptosis inhibitors: Fer-1; DFO; (d, e) Erastin-induced lipid peroxidation (10 μM, 18 hours) in MDA-MB-231 cells was reduced by AZD1390; Lipid peroxidation was assessed by C11-BODIPY staining (d) and quantified as the percentage of lipid peroxidation-positive cells (e). (a, b, and e) Data represent the mean ± SEM. (a, b) 2-way ANOVA and Dunnett’s multiple comparisons. (e) One-way ANOVA followed by Dunnett’s multiple comparison test. (c) Non-parametric Mann-Whitney tests with correction for multiple comparisons. Data represents the median ± IQR. (a-e) n = 6 independent biological replicates. *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001. MDA: malondialdehyde; ATM: ataxia-telangiectasia mutated; LPT1: liproxstatin-1; Fer-1: Ferrostatin-1; DFO: Deferoxamine; SEM: standard error of the mean; ANOVA: analysis of variance; IQR: interquartile range.

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© The Douglas A. Marchuk*, Scott R. Floyd3*, Jen-Tsan Chi* 2026. This is an Open Access article licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, sharing, adaptation, distribution and reproduction in any medium or format, for any purpose, even commercially, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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