CCT2 was highly expressed in HNSCC and related to poor prognosis (IMAGE)
Caption
(A) Up-regulation of 311 genes in HNSCC tumor tissues compared with adjacent normal tissues across the TCGA HNSC, GSE127165, GSE10121, GSE37991, and GSE30784 datasets. (B) CCT2 was highly expressed in tumor tissues compared with adjacent normal tissues. (C) Identification of 45 common prognosis-associated genes through univariate Cox regression analysis in the GSE41613 and GSE42743 datasets. (D) Forest plot of univariate Cox regression analysis for 45 common prognosis-associated genes in the GSE41613 dataset. (E) High expression of CCT2 predicted poor prognosis in GSE41613, GSE42743, and TCGA datasets. (F) Representative immunohistochemistry images illustrating the expression levels of CCT2 and Ki-67 in HNSCC tissues and normal epithelium. (G) Distribution of CCT2 immunohistochemistry scores in tumor and adjacent normal tissues. (H) Western blotting analysis of CCT2 expression was performed on paired tumor and adjacent normal tissues from three HNSCC samples. (I, J) Western blotting and quantitative PCR analysis of CCT2 expression in HNSCC cell lines (HSC-3, CAL-27, and SAS) and normal epithelial cell lines (HOEC and HIEC6). Data were presented as mean ± standard deviation. ∗∗∗p < 0.001 and ∗∗∗∗p < 0.0001 for student's t-test and one-way ANOVA test. CCT2, chaperonin containing TCP1 subunit 2; HNSCC, head and neck squamous cell carcinoma.
Credit
Lin Lu, Yi Shen, Xuerong Li, Yiwei Zhao, Xuefan Zhai, Min Cai, Baicheng Bao, Guiqing Liao, Jianbo Sun
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