Semicentenialea rex as a colony and under the microscope (IMAGE)
Caption
Figure 2 The images show Semicentenialea rex both as a colony and under the microscope. After eight weeks of growth, the fungus forms a small colony on the culture plate. The microscopic images reveal a network of hyphae with so-called clamp connections – structures that connect adjacent cells in the hyphae of basidiomycete fungi. To follow fungal development, the researchers stained the nuclei pink and the cell walls blue. Three types of swollen cells can be observed within the hyphae: basidium-like cells, teliospore- or probasidium-like cells, and intercalary swellings. These structures are examined in greater detail in the following figures.
More scientific: Figure 2. Macro- and microscopic characters of Semicentenialea rex sp. nov. Nuclei stained with propidium iodine (magenta) and cell walls and septa with calcofluor white (cyan), maximum intensity projection of a z-stack in C–D. A Colony growth after eight weeks (petri dish diameter = 90 mm; HU4064, type). B Hypha with clamp connections (arrows) (JH144). C Hyphae with clamp connections (arrows), one with a nucleus in the forming clamp (HU4064, type). D Overview of the hyphae with clamp connections (arrows) and nuclei. Different developmental stages of three types of swellings: basidium-like cells (hereinafter BLC), probasidium/teliospore-like cells (hereinafter TLC), and intercalary swelling can also be seen (HU4064, type), for detailed description of these structures see Figs 3, 4. Rotating 3D rendering of D as Suppl. material 2: movie S1. Scale bars 10 μm.
Credit
Anna Rosling/Uppsala University
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