Evaluation of anaerobic decolorization and biotransformation of Reactive Black 5 (IMAGE)
Caption
A new study shows that a constructed wetland-derived Clostridium sp. strain T4 efficiently decolorizes the azo dye Reactive Black 5 under anaerobic conditions by reductively cleaving its azo bonds. However, this process generates aromatic amine intermediates, including 1,7-diamino-8-hydroxynaphthalene and 4-(ethylsulfonyl)aniline. As these metabolites accumulate, acute toxicity to Daphnia magna increases, showing that visible dye removal alone is not a reliable indicator of wastewater treatment safety.
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Professor Satoshi Soda from Ritsumeikan University, Japan
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