TGFB1 inhibits the growth of pancreatic tumor spheroids derived from PANC-1 cells (first-round screening, 10-d culture) (IMAGE)
Caption
(A) Effects of 365 cytokines (65 families) on PANC-1 tumor spheroid formation in Matrigel after 10 d. (B) Representative cytokine families with the strongest inductive or inhibitory effects on tumor spheroid growth. (C) Representative images of PANC-1 tumor spheroids cultured with or without TGFB1 (10 ng/mL) for 5, 7, and 10 d, captured at 4× or 10× magnification. Scale bar, 10 μm. (D–E) Quantification of tumor spheroids number (D) and size (E) in cultures treated with or without TGFB1 (10 ng/mL) for the indicated time points. Data represent mean ± SD from 5 fields of view and were analyzed by 2-way ANOVA with Sidak post hoc test (*P < 0.05, **P < 0.01, ****P < 0.0001). ANOVA = analysis of variance, IL1 = interleukin-1, SD = standard deviation, TGFB1 = transforming growth factor beta 1, VEGF = vascular endothelial growth factor.
Credit
Credit: Daolin Tang and Rui Kang from UT Southwestern Medical Center, Dallas, USA Image source link: http://dx.doi.org/10.1097/JP9.0000000000000248
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