ANAC050 Expression Patterns Reveal Its Role in Aluminium Stress Response. (IMAGE)
Caption
Expression of ANAC050, examined by reverse transcription-quantitative polymerase chain reaction, in tissues of 4-week-old Columbia ecotype wild-type (WT) seedlings cultivated under normal circumstances (a); expression of ANAC050 in roots of 4-week-old WT seedlings treated with 50 μmol L−1 Al for 0, 0.5, 1, 3, 6, 9, and 12 h (b) and with 0, 5, 10, 25, 50, and 100 μmol L−1 Al for 24 h (c); and expression of ANAC050 in roots (d), root primordia (e), cotyledons (f), inflorescences (g), flowers (h), and siliques (i), as revealed by β-glucuronidasestaining. In panels a, b, and c, internal control TUBULIN was employed, and the expression level of ANAC050 in root without Al treatment was set to 1. Vertical bars indicate standard deviations of the means (n = 4 biological replicates). Bars with different letters are significantly different at P < 0.05. The asterisk * indicates significant difference at P < 0.05.
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