Dermal Microvascular Responses of Human Induced Pluripotent Stem Cell—Derived Skin Organoids to Inflammation and Injury (IMAGE)
Caption
A study in The American Journal of Pathology shows that lab-grown human skin organoids can form complex self-organizing microvascular networks similar to those in native human skin. This figure depicts the features of vascular maturation in human induced pluripotent stem cell—derived skin organoids (SKOs). A—N: Representative images of vascular endothelial cell, mural cell, and extracellular matrix markers from pre— and early hair-forming organoids (days 44 to 54; A—G) and late hair-bearing organoids (days 73 to 105; H—N) are shown. B, F, G, I, L, and N: White arrows indicate putative endothelial lumina. Immunohistochemical profiling was performed on skin organoids collected from three independent experiments. O and P: Quantitative analysis of endothelial and mural cell colocalization (O) and vascular basement membrane protein abundance (P) was performed. A—P: n = 7 early SKOs; n = 13 late SKOs. *P < 0.05, ***P < 0.001, and ****P < 0.0001. AU: arbitrary unit; HSPG: heparan sulfate proteoglycan; PDGFRβ: platelet—derived growth factor receptor-β; α-SMA: α-smooth muscle actin; VECAD: vascular endothelial cadherin.
Credit
The American Journal of Pathology / Sheets et al.
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CC BY-NC-ND