Density Transition of FUS-LCD Visualized by LP-TEM: From Oligomers, Clusters to Dense Phase. (IMAGE)
Caption
Time-lapsed LP-TEM images and schematics illustrating the formation process and highly dynamic nature of dense phase. Left: Top row: Schematic of FUS-LCD clusters and dense phase. Middle row: LP-TEM images of FUS-LCD oligomers; clusters and dense phase are outlined in purple. Bottom row: Binarized images from UNet++ segmentation. Right: Schematic of the dense phase transition process. IDPs/IDRs form oligomers via intermolecular interactions, which then assemble into networked clusters, transitioning to a dense phase above a concentration threshold. In the schematic, blue represents IDP/IDR "stickers" separated by yellow "spacers". "Sticker-sticker" interactions drive oligomerization, network formation, and ultimately, the dense phase. The dense phase schematic is based on LP-TEM images and the "sticker-spacer" model.
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