The SCD1 inhibitor aramchol interacts with regorafenib and metformin to kill tumor cells (IMAGE)
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Figure 5: Metformin increases tumor cell killing caused by [aramchol and regorafenib], which requires macroautophagy and signaling through BID. UM cells and HEP3B cells were transfected with a scrambled siRNA control (siSCR) or with siRNA molecules to knock down Beclin1 and BID. After 24 h, cells were treated with vehicle control, aramchol, regorafenib, metformin or the drugs combined for 24 h. Floating and attached cells from three independent studies were collected and the percentage viability determined using trypan blue exclusion assays (±SD). #p < 0.05 greater than vehicle control; ##p < 0.05 greater than drugs treated with a single agent; ###p < 0.05 greater than drugs treated with two agents; *p < 0.05 less than corresponding values in siSCR transfected cells; **p < 0.05 less than corresponding value in siBeclin1 cells; †p < 0.05 less than corresponding values in siBID cells.
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Copyright: © 2026 Booth et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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